Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetInducible nitric oxide synthase

General description

Mutant of E. coli 0111:B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes. Highly purified preparation.

Mutant of E. coli O111: B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hattori, S., et al. 1995. Biochem. Mol. Biol. Int.35, 177.Mayeux, P.R., et al. 1995. Biochem. Pharmacol.49, 115.Norimatsu, M., et al. 1995. Infect. Immun.63, 1122.Feinstein, D.L., et al. 1993. J. Neurochem.60, 1945.Zhang, Y.H., et al. 1993. Infect. Immun.61, 5044.

Packaging

5 mg in Plastic ampoule

Preparation Note

LPS will form a suspension in H₂O; slight warming and vortexing may be required to achieve complete dispersion in H₂O. Addition of 0.5% triethylamine will also help with dispersion; triethylamine is very basic and can be neutralized with Trix-HCl to avoid hydrolysis of the fatty acid chains.

Reconstitution

Following reconstitution refrigerate (4°C). Stock solutions are stable for up to 1 year at 4°C.

Warning

Toxicity: Harmful (C)